混合製剤中のノスカピンの定量

概要

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Noscapine could be extracted selectively with carbon disulfide from pharmaceutical preparations, and determined conveniently by the infrared spectrophotometry. The most intense absorption band at 1770 cm-1 was chosen as the key band. The recommended procedure is as follows : Method A : A sample which contains about 50 mg of noscapine is placed in a glass-stoppered flask, add 20 ml of carbon disulfide, and shake the flask for 10 minutes. Filter the contents of the flask through a glass-filter, and the residue is treated similarly with 10 ml of the same solvent three times. Filtrates are put together and diluted to 50 ml with carbon disulfide. Measure the absorbances of this solution (ET) and a standard solution (1 mg/ml) (ES) against a solvent blank, and estimate the amount of noscapine from the ratio of ET to ES. Method B : A sample which contains about 10 mg of noscapine is placed in a centrifuge tube, add 25 ml of carbon disulfide, shake the tube for 10 minutes, and centrifuge the contents of the tube. After removing a supernatant liquid the same procedure is repeated three times. The whole supernatant liquid is put together, condensed to about 5 ml in vacuo, and diluted to 10 ml with carbon disulfide. Measure the absorbances and estimate the amount of noscapine similarly as mentioned above.
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