混合製剤中の塩酸キニーネの定量

概要

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Quinine hydrochloride (I) reacts with Bromophenol Blue in an acid medium (pH 6.2) to give a red color. This chromogen can be extracted into benzene-chloroform (1 : 1) and shows an absorption maximum at 585 mμ. The recommended analytical procedure is as follows. Take a sample containing 40 mg of (I) in a stoppered flask. Add 5 ml of dil. HCl and 50 ml of water, and warm on a water bath at 60°C. Cool to room temperature. Transfer the solution to a 100 ml volumetric flask, and add water to make up to 100 ml. Centrifuge this solution, and pipet 50 ml of the supernatant into a 100 ml beaker. Add 10 ml of 0.5M KH2PO4 solution and adjust pH to 6.2 with 0.1N NaOH. Transfer the solution to a 100 ml of volumetric flask, and add water to make up exactly to 100 ml. Transfer a 2 ml aliquot into a 50 ml glass-stoppered centrifuge tube and add 2 ml of buffer (pH 6.2), 1 ml of 0.06% Bromophenol Blue solution and 20 ml of benzene-chloroform (1 : 1). Shake for about 5 min, centrifuge and allow to settle the benzene-chloroform layer. Measure the absorbance at 585 mμ (ET) against benzene-chloroform. At the same time, measure the absorbance of the standard solution (ES) and determined the amount of (I) by ET/ES×1.100. Quinine sulfate was analysed by the same method, the value being caluculated by ET/ES×1.048.
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