カップ法によるビタミンB群の微生物定量にかんする基礎的研究 : (XVI) Lactobacillus caseiを用いるカップ法によるビタミンB_2の定量

概要

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A microbiological assay for riboflavin by cup-plate method using Lactobacillus casei as test organism was attempted. In the case of L. casei assay, a strain having a great sensitivity to riboflavin should be chosen, since most of the laboratory strains maintained by monthly transfers have almost lost their necessity for the vitamin. Under our assay condition, a clear, sharp and reproducible growth zone was obtained, and in the statistical analysis of the results the maximum errors of the estimate were below 15% indicating a high precision of the method. The riboflavin contents in several biological materials determined by plate method were nearly equal to those obtained by both turbidimetric and fluorometric ones. Also, the recoveries of the added vitamin were satisfactory enough except for a few samples from vegetable source. These results may support that the cup-plate assay for riboflavin using L. casei as test organism is a valuable analytical method, though it is relatively low in sensitiveness.
日本ビタミン学会の論文
1970-11-25