菌糸懸濁液の攪拌における菌糸に与える衝撃の強さを表わす基準について : 糸状菌の液内培養における菌糸に与える攪拌の影響に関する研究 (第3報)

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On agitation of mycelial suspensions of Mucor javanicus and Rhizopus javanicus at a constant impeller speed, intracellular nucleotides leaked from mycelia to water in direct proportion to agitation time. The rate of leakage determined was dependent on the agitator speed.1. On agitation of mycelial suspensions, and in aeration-agitation cultures of the mold, RNA-related nucleotides, mostly monomucleotides, were caused to leak by the mechanical agitation.2. The phenomenon was not caused by the fracture of mycelia.3. The composition of the nucleotides was the same regardless of culture apparatus and culture age. From the above results, the rate of leakage of intracellular nucleotides at a mycelial concentration of 100mg dry weight per 100 ml, Δ_<260>, was proposed as a standard for the intensity of agitation shock on mycelia on agitation of mycelial suspension.This standard correlated satisfactorily with the impeller tip velocity when compared with the modified Reynolds number and power consumption per unit volume. Further, the number of baffile plates and the viscosity of the mycelial suspension affected Δ_<260>.The values of Δ_<260> for the jar fermenter and the shaking flask under conditions of equal oxygen supply were determined. The intensity of shock on mycelia in the jar fermenter was found to be about 2 to 3 times that for the flask. On the basis of these results, it was supposed that the intensity of shock on mycelia in the jar fermenter and the flask in practical sulture would also be as mentioned above in early phase of growth.
社団法人日本生物工学会の論文
1975-01-25

菌糸懸濁液の攪拌における菌糸に与える衝撃の強さを表わす基準について : 糸状菌の液内培養における菌糸に与える攪拌の影響に関する研究 (第3報)

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