機械的攪拌に対して生理的活性維持の菌糸強度を表わす指標について : 糸状菌の液内培養における菌糸に与える攪拌の影響に関する研究 (第5報)

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Leakage of intracellular nucleotides on agitation of mycelial suspension was examined using 18 strains of filamentous microorganisms which included species of Mucor, Rhizopus, Aspergillus, Penicillium, Neurospora, Pircularia, Cephalosporium and Streptomyces. Intracellular low molecular nucleotides were found to leak on agitation of mycelial suspensions, as reported for Mucor javanicus in a previous paper, and this was considered to be a common phenomenon in filamentours microorganisms.From the knowledge that the growth of Mucor javanicus is influenced according to the amount of intracellular nucleotides leaked by agitation, an index, R, representing the mycelial strentgh to maintain physiological activity on mechanical agitaiton, was proposed. R was defined as the ratio of the leakage rate of low molecular uncleotides under fixed agitation comditions to the total amount of nucleic acid-related substances contained in the mycelia. With nominal mycelial lengths above 300μm, R invreased in proportion to the length. From the comparison of R among molds of the same nominal mycelial length, the mycelial strength of Penicillium species was considered to be very weak.In aeration-agitation culture of Mucor javanicus, it was found that seed mycelia under 300μm in nominal length and a low agitation speed were necessary to obtain normal growth of the mold. This finding was explained by the leakage of nucleotides during culture.
社団法人日本生物工学会の論文
1975-01-25

機械的攪拌に対して生理的活性維持の菌糸強度を表わす指標について : 糸状菌の液内培養における菌糸に与える攪拌の影響に関する研究 (第5報)

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