グルタミン酸醗酵の連続化に関する研究 : (第3報)多段連続醗酵の基礎的実験

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To investigate the glutamic acid production by continuous multi-stage fermentation, it was decided to begin experiments using three seperate vessels in series. The first vessel was used only for propagating active cells. to provide the latter vessels. The second vessel was used to provide sufficient retention time for the completion of the growth of cells and fermentation of the greater part of the sugar. The third vessel was used to provide the additional retention time necessary to complete the fermentation. The experiments were carried out with the help of the information gained from previous experiments in contamination control and the continuous fermentation in the growth phase. The results of continuous fermentation by the multi-stage system are shown in Figures 4 and 5. From these experiments the following findings were obtained. 1. The most important problem for continuous operation is to control the transitional phenomena which appear markedly in the first fermentor soon after the start of continuous feeding. 2. A major cause for the lowering of productivity in the continuous system is that the level of the fermentation phase in the first fermentor is much lower than that of the second fermentor. 3. The amount of biotin supplement must be adjusted during the continuous feeding to obtain a high production yield. It was also found that manganese became a growth limiting factor in this series of experiments.
社団法人日本生物工学会の論文
1963-12-15

グルタミン酸醗酵の連続化に関する研究 : (第3報)多段連続醗酵の基礎的実験

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