グルタミン酸醗酵の連続化に関する研究(第2報) : 増殖相における定常醗酵の雑菌汚染管理の検討と定常維持因子の発見

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I. For a consideration of infection control in continuous fermentation based on the analysis of infectant growth, growth rate constant (k) and lag time (L) were measured for the fermentation media containing drug mixtures as shown in a previous paper.The comparison of k and L are shown in Table 1. The infectants used in this experiments were isolated from the fermentation processes in a factory and were highly resistant to drugs in the nutrient broth. From Table 1 the following facts were observed : 1 In the presence of a drug mixture, lag time of infectants was long in the production medium, but not in the seed culture medium and it was generally impossiblie to reduce k velues in both of the media. 2. It was also impossible to reduce k values remarkably in the prodution medium with the progress of fermentation as recognized in Table 2.From these facts the authors came to the conclusion that continuous fermentation may be continued for a long period using the drug mixture if the seed culture and feeding medium are well controlled.II. In the continuous fermentation in the growth phase it was found that biotin is a limiting factor for cell growth, and the biotin supplement must be put into the fermenting broth continuously to maintain a fairly high cell concentration. The amount and startiing times for adding biotin were important. In this experiment 0.1μg biotin was added to 2.5l broth hourly and the addition of biotin was started prior to the decrease of cell concentration. These experiments are registed in Figures 4〜8.
社団法人日本生物工学会の論文
1963-09-15

グルタミン酸醗酵の連続化に関する研究(第2報) : 増殖相における定常醗酵の雑菌汚染管理の検討と定常維持因子の発見

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