糸状菌の通気攪拌培養における菌糸内ヌクレオチド類の漏出現象と漏出物質の同定

概要

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In the culture of Mucor jawanicus in synthetic medium with agitation and aeration, nucleic acid related substances were accumulated in the culture liquid, but the cutting of mycelia was not observed. in a seried of quantitatibe analyses, the substances were found to be RNA related low molecular nucleotides. They were further fractionated by anion exchange resin, Dowex 1-X8. Most of them were found to be mononucleotides (3'-CMP, 3'-AMP, 3'-GMP and 3'-UMP) by paper chromatography and ultravilet absorption spectra. Despite having the same cultural environment, differences in growth behavior and accumulation of the nucleotide were found between flask and jar fermenter cultures. On the other hand, no differences in composition of the nucleotides or the quantitative ratio of each nucleotide to total nucleotides were found, regardless of culture apparatus and culture age. Also, the compsotion of the nucleotides was the same as that of the nucleotides leaked during the agitation of the mycelial suspension reported in the previous paper. From these findings, it was detected that the nucleotides were not so-called fermentation products, but substances leaked from mycelia by agitation.
社団法人日本生物工学会の論文
1974-09-25