グルタミン酸醗酵の連続化に関する基礎的研究 (第1報) : 薬剤を利用する雑菌汚染管理の基礎実験

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The utilization of dergs for glutamic acid fermentation was investigated to overcome the infection troubles in continuous operation. The growth-inhibiting power of drugs over infectants was examined in a medium containing glucose, meat extract, corn-steep liquor, urea and some salts. The infectants were isolated from soil samples during our search for glutamate-producing bacteria.Phenols, nitrofurans, sulfonamides, surface active substances, antibiotics, dyes, formalin and vitamin K_3 were found to be effective. The rate of growth inhibition by some drugs is shown in Fig. 1 and 2.As glutamate-producing bacteria were also sensitive to these drugs, they had to be cultured repeatedly on the nutrient agar slant containing a drug until they could grow normally. By this method, the trained strains highly resistant to drugs were obtained.The difference of drug adaptation between two strains is shown in Table 1. The production of glutamic acid by these trained strains in shaking culture, however, was not successful when the concentration of a drug in the medium was high enough to inhibit the growth of most contaminants. But it was found that such a influence of a drug observed in a high concentration upon the glutamate production could be removed by applying drugs in combination as shown in Table 2. The inhibiting power of drug mixtures is depicted in Fig. 3.Training of glutamate-producing organisms through repeated cultivation more than twenty times in media added with drug mixture was necessary for obtaining successful results of fermentation.
社団法人日本生物工学会の論文
1963-03-15

グルタミン酸醗酵の連続化に関する基礎的研究 (第1報) : 薬剤を利用する雑菌汚染管理の基礎実験

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