味噌麹菌に關する研究 (第4報) : 味噌麹菌の榮養變異株について

概要

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1) 10 biochemical mutants (nutritionally deficient mutants) showing growth on Koji extract but no growth on CZAPEK's solution were obtained from Aspergillus oryzae No. 15 strain for Miso making by irradiation with ultraviolet ray.2) These strains incapable to grow on the minimal medium were tested with five stock solutions. These solutions contain the following supplements or substitutes ; 1) 2% yeast extracts, 2) hydrolyzed casein, 3) vitamin solution added to the minimal medium, 4)(NH_4)_2SO_4 substituted for NaNO_3,5)NaNO_2 substituted for NaNO_3 in the minimal medium.3) After experiments, 2 strains requiring glycine, 2 strains requiring leucine, 4 strains requiring lysine and 2 double gene mutants requiring amino acid were found.4) Biochamical mutants requiring vitamins and bases were not obtained .
社団法人日本生物工学会の論文
1953-07-15