微生物のタンニン酸加水分解酵素に関する研究 : (第1報)生産菌の分離同定と酵素生産のための培養条件の検討

概要

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From about eighty strains tested for enzyme production, the superior, strains No. 7 and No. 37,were obtained. Enzyme activity was determined by acid titration using sodium hydroxide.Both obtained strains were identified as Asp. oryzae.The enzyme produced by these strains was able to hydrolyze every position of ester bond of tannic acid. Consequently, in complete enzymatic hydrolyzate of tannic acid, only glucose and gallic acid were detected.Conditions for the cultivation of strain No. 7 for the enzyme production were investigated and the optimal conditions were decided as follows : Tannic acid (J.P.) 20g, NH_4Cl 2g, KH_2PO_4 2〜3g, MgSO_4・7H_2O 1g, AlCl_3・6H_2O 0.01〜0.05g, distilled water 1.0 l; initial pH 6.0 (adjusted with 2N-NaOH); flash sterilized at 120°50 ml of medium/500 ml vol. flask, 120 oscil./min., 70〜120 hours cultivation, precultured on an agar medium of an same composition.The plate culture was prepared as follows : After the ager medium (without tannic acid) was spread on a filter paper disk placed to cover entirely the bottom of a petri dish, concentrated tannic acid solution was spread to permeate the agar gel.
社団法人日本生物工学会の論文
1967-03-25