微生物によるL(+)酒石酸の生成に関する研究 : (第2報)基礎的培養条件

概要

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Fundamental culture conditions for the production of tartaric acid by the isolate, Gluconobacter suboxydans 2026Y2,were studied. The optimum temperature for the acid production was 26℃ and any higher temperatures were more unfavorable than lower temperatures. The amount of CaCO_3 in the fermentation medium had much influence on the yield of tartaric acid. The amount of CaCO_3 a little less than those equivalent to flucose (20% flucose) resulted in the largest yield. A moderate amount of NH_3-N viz. 0.15% of NH_4Cl in the medium was the best and an increase in quantity of nitrogen source decreased the yield considerably. Among various metal ions tested, the effect of manganese ion was remarkable. An addition of only 0.2mM of manganese sulfate to the fermentation medium was proved to be sufficient, while most of the metal ions added at an equal concentration were useless or inhibitory. The effect of vanadate ion was also noticeable. An addition of 0.85mM of NH_4VO_3 to the fermentation medium remarkably increased the yield of tartaric acid, but an increase in concentration of vanadate decreased the yield significantly. A synthetic medium proved to be most suitable for the production of tartaric acid by shake flask fermentation. The greatest yield obtained from 6-day fermentation was 8.5% based on added glucose.
社団法人日本生物工学会の論文
1971-02-25