微生物のフィターゼに関する研究 : (第2報)Asp. terreus Thom (No. 9A-1)の固体培養におけるフィターゼの生産条件と粗酵素の性質

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The optimum culture conditions for phytase production by Asp. terreus Thom (No.9A-1) isolated from the soil were investigated by a solid culture and the following results were obtained : Rice bran was a suitable medium for the enzyme production and a high enzymatic activity was aquired when 35 g water was added to 100 g rice bran. The more water was added, the more myceria grew. However with regard to enzyme production, the effect was just reverse.By an addition of ammonium salts to a basal medium, enzyme production was significantly increased.Phytase thus produced was stable with pH between 3.0 and 6.0 and at a temperatures below 40℃. The optimum pH for the enzymatic activity was 3.0-3.6,and the optimum temperature ranged from 60 to 65℃. The enzyme hydrolyzed more than 95% of the phosphate linkages of Na-phytate.
社団法人日本生物工学会の論文
1968-11-25