クエン酸醗酵に関する研究 : (第9報)微量金属のクエン酸生産に及ぼす影響

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1. A profound difference was observed in growth and citric acid production between sucrose- and dextrose-salts medium.2. The differences was found to be due to the trace metals contained as impurties in these sugars used. Addiition of sucrose ash to the dextrose-salts medium previously treatd with Dowex Chelating Resin A-1 resulted in the same good yield of citric acid as that with sucrose-salts medium.3. Addition of sucrose ash to dextrose-salts medium previously made free from manganese brought about nearly the same yield (72.5%) as that with sucrose-salts medium. But, the good yield as above was not obtained with dextrose-salts medium made free from iron, zinc or copper instead of manganese.4. After being treated with chelating resin, trace metals of dextrose and sucrose used were determined as follows.[table]5. Addition of 0.1 to 0.2mg each of iron and zinc to a liter of sucrose- or dextrose-salts medium previously treated with chelating resin gave almost equally good yields (80 to 85%). Accordingly, it appears that little difference exists in the effectiveness as carbon sources for citric acid production between sucrose and dextrose.6. Citric acid formation from different carbohydrate from which trace metals were removed with chelating resin was compared. It was shown that sucrose, fructose, mannose and dextrose were excellent, xylose and mannit were good, and galactose was poor. Arabinose was not assimilable.7. With commercial cerelose as carbon soure, the yield of citric acid could be raised by about 20% (e.g. to 79.6%) when 20% of the sugar was used at first after being treated with resin and the remainder (80%) was fed to the 48 hour culture in which growth had attained nearly to the stationary level.
社団法人日本生物工学会の論文
1962-04-15

クエン酸醗酵に関する研究 : (第9報)微量金属のクエン酸生産に及ぼす影響

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