クエン酸醗酵に関する研究(第3報) : 酸生産に及ぼす培地と培養温度の関係並びに培養温度変化に就いて

概要

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1) In the production of citric acid from 25% molasses with 3% methanol, there were no great differences in the yields of acid between the concentrations of NH_4NO_3 from 0.1 to 0.25% except and extreme low (0.05%) and high (0.30%) concentrations which retarded the acid production.2) The type of nitrogen compounds tested (NH_4NO_3,NH_4H_2PO_4,(NH_4)_2HPO_4,(NH_4)_2SO_4,(NH_2)_2CO, (NH_4)_2CO_3 and CSL) was of no great influence on the amount of citric acid formed from cane molasses with 3 per cent methanol.3) The optimum fermentation temperature depends on the concentration of NH_4NO_3 ; at the concentrations of 0.3 and 0.2 per cent, the optimum is 25℃ and at 0.1 and 0.05 per cent it is 28℃.4) So long as the fermentation temperature is kept at 33℃ throughout the fermentation, the yield of acid is quite low. But, when transferred to 28 or 25℃ after one or two days from the time of incoulation, the yield was considerably increased.Time of temperature changes is a very important factor for increasing the acid production. The highest yield of acid was obtained by the fermentation which was started at 28℃ and transferred to 25℃ after 48 hrs.
社団法人日本生物工学会の論文
1960-10-15