クエン酸醗酵に関する研究(第1報) : 糖蜜培地のクエン酸醗酵に於いてメタノール, エタノールの酸生成に及ぼす影響

概要

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With a cane molasses medium in a shake flask, effects of methanol and ethanol on the production of citric acid by Asp. niger were studied and the following results were obtained.1) Methonal grealty stimulates the production of citric acid and the highest yield of the acid was obtained with 3 per cent of methanol.2) Ethanol also stimulates the production of citric acid, but at 3 per cent it was much less effective than methanol and at 1 and 2 per cent somewhat inhibitory.3) The use of ethanol leads to a high pH value, slow utilization of sugar and less residual amount of alcohol than that of methanol.4) The optimum time of methanol addition is immediately before inoculation. In case of the addition of methanol after one day from the time of inculation, only 57 per cent of citric acid obtained from the control was produced and little effect toward increasing citric acid production was observed thereafter.5) Judging from the effect of the time of methanol addition on the production of citric acid and the growth rate, methanol should be required during the initial growth stage. Methanol seems to play a part of enzyme formation by the mold for the production of citric acid from molasses.
社団法人日本生物工学会の論文
1960-10-15