クエン酸醗酵に関する研究(第5報) : メタノール添加糖蜜培地のクエン酸醗酵に於ける蓚酸併生に就いて

概要

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1) Methanol stimulates the production of citric acid from cane molasses. No oxalic acid was formed as by-product in fermentations with methanol added in the concentrations of 1 and 2 per cent that gave the highest yield of citric acid, but a high NH_4NO_8 content of the culture medium had a tendency to yield more oxlic acid.2) In the citric acid production by Asp. niger N-584,the formation of oxalic acid depends on the kind of media. Both the synthetic and hte semi-synthetic medium with a small quantity of C.S.L. and 3 per cent of methanol, which were suited to the citric aicd procution, didn't give oxalic acid.3) In the production of citric acid from cane molasses, the addition of more than 3 per cent methanol within 12 hrs after inoculation caused the oxalic acid formation. Therefore, it is desirable to use methanol as little as possible at the early stage of fermentation, in order to reduce the oxalic acid formation. But no less than 2.5 per cent of methanol should be added in the early growth phase to stimulate the citric acid production.4) The formation of oxalic acid proceeds parallel with the production of citric acid durig the fermentation.
社団法人日本生物工学会の論文
1960-11-15