クエン酸醗酵に関する研究(第4報) : 培養条件の酸生産に及ぼす影響

概要

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The effects of culture conditions on the production of citric acid from cane molases were investigted and the results obtained were as follows.1) The use of seed culture having a low pH brought about by the conversion of sugar to acid resulted in a low yield of acid. It is desirable to culture the seed under a mild condition.2) In a 5l jar fermenter, the highest yield of acid was obtained, when the culture was maintained a 600 r.p.m., and 0.5l of air/l of medium/min. for 48 hrs after inoculation, and thereafter at 1.0l of air/l of medium/min. As for the concentration of NH_4NO_3 in the culture medium, 0.2 per cent was better than 0.15 per cent.3) In submerged culture in a 5l jar fermenter, a concentration of 1.0 per cent was preferable to 1.5 per for methanol to be added after one day from the time of inoculation, when the concentratons of NH_4NO_3 in culture media were low (0.15,0.2%). On contrary, at a higher concentration of NH_4NO_3 (0.25%), 1.5 per cent of methanol brought about a better effect.
社団法人日本生物工学会の論文
1960-11-15