クエン酸醗酵に関する研究 : (第8報)微量金属のクエン酸生産に及ぼす影響

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1. The yield of 74% was obtained by adding 0.1m g of iron to a liter of sucrose-salts medium. Under the same conditions, there observed little change in yield by further addition of 2.5 mg of zinc. Addition of 3 or 30 μg of manganese resulted in a decrease in yield by about 8 or 55% resp.2. The inhibitory effect of manganese depends on the content of iron in the medium. At a level of 10μg of manganese, addition of 0.1 or 1.0 mg of iron caused a decrease in yield by about 36 or 94%, resp., as compared with experiments without manganese.3. In the absence of iron, addition of 2.5 mg of zinc gave the yield of 60.7%, though the amount of residual sugar was coinsiderable, while with the additional 5.0 mg of manganese, the yield of 45.5% was obtained. It appears that the excess of zinc does not strengthen the inhibitory effect of manganese on acid production if iron is not present.4. In sucrose-salts medium having sufficient amount of iron and zinc, remarkable accumulation of citric acid could not be observed even when the growth was controlled by limiting the contents of NH_4NO_3,MgSO_4・7HO and KH_2PO_4 in the medium.5. Addition of methanol (3% v/v) was effective in suppressing growth to some extent and stimulating citric acie production in sucrose-salts medium containing iron in sufficient amount. But, in the case of Asp. niger 72-4,the similar effect on acid production was observed without affecting growth.6. The addition of methanol (3% v/v) stimulated citric acid production in sucrose-salts medium containing iron, zinc and manganese in amounts sufficient to suppress citric acid production almost completely.7. A profound difference in the effects of trace elements upon growth and citric acid production was observed between two strains of Asp. niger, N 584 and 72-4.
社団法人日本生物工学会の論文
1962-04-15

クエン酸醗酵に関する研究 : (第8報)微量金属のクエン酸生産に及ぼす影響

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