SODを欠損した大腸菌に及ぼす変動磁場の影響

概要

論文の詳細を見る
In this study, Escherichia coli mutant IM303 was used to detect the effect of pulsed of magnetic fields through reactive oxygen species. IM303 is a mutant defective in the superoxide dismutases Fe-SOD and Mn-SOD. The strength of the exposed magnetic field was 0.06, 0.1, or 0.13 T. Trains of 10 or 5 pulses of magnetic field at intervals of 0.05s were applied at 1 Hz. The growth of the mutant was detected by measuring the light absorbance at 600nm every hour. After six hours of exposure, cell were centrifuged and two-dimensional gel electrophoresis of the proteins in E. coil was performed. Two-dimensional gel electrophoresis data revealed lost protein in the exposed samples of IM303. Their growth rate did not change with exposure. The results of this study indicate that the synthesis of proteins that do not change the cell cycle length has been altered. However, lethal damage to IM303 did not occur.
社団法人日本磁気学会の論文
1998-04-15