連続磁気刺激による熱ショック蛋白質発現の変化

概要

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In this study, we focused on the biological of repetitive-pulsed magnetic stimulation. Using the Swiss mouse embryo-derived fibroblast cell line (NIH3T3) and ret-II (a colon cancer-derived oncogene)-transformed cells (Ret-II) as a model of normal and malignant cells, changes in the expression of heat shock protein 70(HSP70) were investigated by Western-blotting analysis. The cells were exposed to train-pulsed magnetic fields at 50 Hz, and the waveform of each pulsed field was a biphasic shape with a 200 μs duration. The magnetic field intensity was 17 Mt. As a result, the expression of HPS70 in both NIH3T3 and Ret-II cells did not change at 37℃, but at 40℃, the expression in both cells increased after stimulation for 3 h and 6 h but decreased after stimulation for 12 h. When incubated at 42℃, an increase in the expression of HSP70 due to the increase in temperature was observed. It is concluded that, under normal conditions, cells are not affected by magnetic stimulation, but under adequate stress, magnetic stimulation is also a stressful stimulus.
社団法人日本磁気学会の論文
1998-04-15