Selective Solvent Extraction of Ruberythric Acid from Madder Roots and Subsequent Hydrolysis with β-Glucosidase
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概要
- 論文の詳細を見る
Selective solvent extraction of anthraquinones (alizarin and lucidin) and their primeverosides (ruberythric acid and lucidin-3-o-primeveroside) from madder roots could be achieved using two-phase extraction with chloroform (CHCl_3) and potassium hydroxide aqueous solution (KOH_<aq>). Ruberythric acid extracted to the aqueous phase could be selectively and completely converted to alizarin within 360 min by enzymatic hydrolysis with β-glucosidase at 50℃ and at pH 5,reducing the inhibitory effect on the reaction caused by alizarin, which is not extracted in appreciable amounts into the aqueous phase, without appreciable hydrolysis of lucidin-3-o-primeveroside. It was also found that ruberythric acid extracted from madder hairy roots by CHCl_3-KOH_<aq> two-phase extraction was efficiently converted to alizarin in the same manner.
- 公益社団法人日本生物工学会の論文
- 1996-06-25
著者
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東稔 節治
大阪大学大学院 基礎工学研究科 化学系専攻化学工学分野
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Tone Setsuji
Department Of Chemical Engineering Faculty Of Engineering Science Osaka University
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Taya Masahito
Department Of Biotechnology Graduate School Of Engineering Osaka University
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正脇 輝之
大阪大学大学院基礎工学研究科 化学系専攻化学工学分野
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Masawaki Teruyuki
Department Of Chemical Engineering Faculty Of Engineering Science Osaka University
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