NADH-Dependent Inhibition of Branched-Chain Fatty Acid Synthesis in Bacillus subtilis

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概要

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• Addition of NADH to crude but not to pure branched-chain α-keto acid decarboxylase decreased the CO_2 production from α-keto-β-methylvalerate (KMV) suggesting the presence of an NADH dependent inhibitor in the crude enzyme from Bacillus subtilis. This NADH-dependent decarboxylase inhibitor was purified to homogeneity by a fase protein liquid chromatography system. The purified inhibitor was identical with leucine dehydrogenase as to N-terminal amino acid squence (35 residues) and molecular weight, and catalyzed the oxidative deamination of three branched chain amino acids (BCAAs), valine, leucine, and isoleucine. The decarboxylase inhibitor was therefore identified as leucine dehydrogenase. A decreased substrate availability caused by leucine dehydrogenase thus reasonably accounted for the NADH dependent inhibition of the decarboxylation. In trun, the observation that leucine dehydrogenase competes with the decarboxylase for branched-chain α-keto acid (BCKA) suggested an involvement of this enzyme in the branched chain fatty acid (BCFA) biosynthesis. This view was supported by the observation that addition of NAD to crude fatty acid synthetase increased the incorporation of isoleucine into BCFAs. Pyridoxal-5'-phosphate and α-ketoglutarate, cofactors for BCAA transaminase, modulated BCFA biosynthesis from isoleucine in vitro, suggesting also the involvement of transaminase reaction in BCFA biosynthesis.

• 社団法人日本農芸化学会の論文
• 1998-04-23

著者

• OKU Hirosuke

  Laboratories of Applied Biochemistry, University of The Ryukyus

• CHINEN Isao

  Laboratories of Applied Biochemistry

• Oku Hirosuke

  Center Of Molecular Biosciences Tropical Biosphere Res. Center Univ. Of The Ryukyus

• IWASAKI HIRONORI

  Division of Molecular Biotechnology, Center of Molecular Bioscience, University of the Ryukyus

• IWASAKI Hironori

  Laboratory of Applied Biochemistry, Faculty of Agriculture, University of The Ryukyus

• FUJITA Keisuke

  Laboratory of Applied Biochemistry, Faculty of Agriculture, University of The Ryukyus

• NOMOTO Tomoko

  Laboratory of Applied Biochemistry, Faculty of Agriculture, University of The Ryukyus

• SUZUKI Kiyoshi

  Laboratory of Applied Biochemistry, Faculty of Agriculture, University of The Ryukyus

• Chinen Isao

  Laboratories Of Applied Biochemistry University Of The Ryukyus

• Chinen I

  Univ. The Ryukyus Okinawa Jpn

• Chinen Isao

  Laboratory Of Applied Biochemistry Faculty Of Agriculture University Of The Ryukyus

• Chinen Isao

  Laboratories Of Applied Biochemistry Faculty Of Agriculture University Of The Ryukyus

• Iwasaki Hironori

  Division Of Molecular Biotechnology Center Of Molecular Bioscience University Of The Ryukyus

• Oku Hirosuke

  Laboratories Of Applied Biochemistry University Of The Ryukyus

• Nomoto Tomoko

  Laboratory Of Applied Biochemistry Faculty Of Agriculture University Of The Ryukyus

• Fujita Keisuke

  Laboratory Of Applied Biochemistry Faculty Of Agriculture University Of The Ryukyus

• Oku H

  Division Of Molecular Biotechnology Center Of Molecular Bioscience University Of The Ryukyus

• Oku Hirosuke

  Division Of Molecular Biotechnology Center Of Molecular Biosciences University Of The Ryukyus

• Oku Hirosuke

  Laboratory Of Applied Biochemistry Faculty Of Agriculture University Of The Ryukyus

• Suzuki Kiyoshi

  Laboratory Of Applied Biochemistry Faculty Of Agriculture University Of The Ryukyus

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