cDNA Cloning and Molecular Analysis of Papaya Small GTP-binding Protein, pgp1
スポンサーリンク
概要
- 論文の詳細を見る
In the course of papaya EST collection, one clone (pRA4-3) encoding partial sequence of papaya small GTP-binding protein gene, pgp1, was obtained . Based on the sequence information of pRA4-3, the entire coding region of pgp1 was cloned using the 3RACE PCR technique. ORF of pgp1 is 636bp long and deduced molecular weight of the protein is 23311. Phylogenetic analysis showed that PGP1 belongs to YPT/RAB group of the small GTP-binding protein and is a homologue of RAB2. Southern analysis showed that there are several pgp1- related genes in papaya genome. Northern analysis showed that pgp1 was expressed equally in stems of seedlings that were grown under light and dark conditions. This result shows that PGP1 is not involved in the phytochrome-mediated signal transduction as an auxin signal transducer in stems of papaya seedlings.
著者
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TANAKA Hiroshi
Department of Pediatrics, Hirosaki University School of Medicine
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KAYANO Toshiaki
Department of Cell Biology, National Institute of Agrobiological Resources
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Chinen Isao
Laboratories Of Applied Biochemistry University Of The Ryukyus
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Chinen I
Univ. The Ryukyus Okinawa Jpn
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Chinen Isao
Laboratory Of Applied Biochemistry Faculty Of Agriculture University Of The Ryukyus
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Chinen Isao
Laboratories Of Applied Biochemistry Faculty Of Agriculture University Of The Ryukyus
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Chinen Isao
Department Of Agricultural Chemistry University Of The Ryukus
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TARORA Kazuhiko
Okinawa Prefectural Agricultural Research Center
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Kayano Toshiaki
Department Of Biotechnology National Institute Of Agrobiological Resources
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Tarora Kazuhiko
Okinawa Prefectural Agricultural Experiment Station
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Tanaka Hiroshi
Department Of Cardiology Jichi Medical School
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Urasaki Naoya
Okinawa Prefectural Agricultural Experiment Station
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Terauchi Ryohei
Iwate Biotechnology Research Center
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Tokumoto Masakazu
Okinawa Prefectural Agricultural Experiment Station
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URASAKI Naoya
Iwate Biotechnology Research Center
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BAN Yasunori
Department of Biotechnology, National Institute of Agrobiological Resources
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Ban Yasunori
Department Of Biotechnology National Institute Of Agrobiological Resources
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Terauchi R
Iwate Biotechnology Research Center
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Tanaka Hiroshi
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hoshi University
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Kayano Toshiaki
Department of Biotechnology, National Institute of Agrobiological Resources
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