Cloning and Functional Expression of Cycloartenol Synthases from Mangrove Species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce

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概要

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• To obtain cDNAs encoding oxidosqualene cyclase (OSC), we cloned two cDNAs, KcCAS and RsCAS, from roots of Kandelia candel (L.) Druce and leaves of Rhizophora stylosa Griff. by homology based PCR method respectively. The deduced amino acid sequences of both OSCs showed 82% homology to cycloartenol synthases from Lotus japonicus (OSC5) and Ricinus cummunis (RcCAS), suggesting that these are cycloartenol synthases of K. candel and R. stylosa. The genes obtained were expressed in a lanosterol synthase deficient Saccharomyces cerevisiae (ERG7) strain, GIL77. GC–MS analysis identified the accumulated reaction product in the yeast transformant to be cycloartenol, indicating that both KcCAS and RsCAS encode cycloartenol synthase.

• 社団法人 日本農芸化学会の論文
• 2007-07-23

著者

• BASYUNI MOHAMMAD

  United Graduate School of Agricultural Sciences, Kagoshima University

• BABA SHIGEYUKI

  Tropical Biosphere Research Center, University of the Ryukyus

• Baba Shigeyuki

  Tropical Biosphere Research Center University Of The Ryukyus

• Oku H

  Division Of Molecular Biotechnology Center Of Molecular Bioscience University Of The Ryukyus

• Oku Hirosuke

  Division Of Molecular Biotechnology Center Of Molecular Biosciences University Of The Ryukyus

• Oku Hirosuke

  Division Of Molecular Biotechnology And Center For Molecular Bioscience University Of The Ryukyus

• Oku Hirosuke

  Laboratory Of Applied Biochemistry Faculty Of Agriculture University Of The Ryukyus

• TSUJIMOTO Etsuko

  Graduate School of Agriculture, Faculty of Agriculture, University of the Ryukyus

• Basyuni Mohammad

  United Graduate School Of Agricultural Sciences Kagoshima University

• Tsujimoto Etsuko

  Graduate School Of Agriculture Faculty Of Agriculture University Of The Ryukyus

• Oku Hirosuke

  Div. Of Molecular Biotechnology And Center Of Molecular Bioscience Univ. Of The Ryukyus

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