Improved Bioassay Method for Plant Transformation Inhibitors

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概要

• 論文の詳細を見る

• A convenient and quantitative bioassay method for evaluating the efficiency of plant transformation by Agrobacterium tumefaciens is important to search plant transformation inhibitors, possible biochemical probes for study on its mechanism. Our previously reported method, in which the plant transformation had been detected by the expression of β-glucuronidase in transformed plants, was improved. The difference between the previous and the improved methods is the use of suspension-cultured cells of Ageratum conyzoides as the host plant instead of Nicotiana tabacum BY-2 ; this alteration of the host enabled us to measure the β-glucuronidase activity in plant cells not only fluorometrically but also colorimetrically. The enzyme activity expressed in the cells of A. conyzoides was nearly 100 times higher than that of N. tabacum BY-2,and was enough for detection by colorimetric measurement. The method, therefore, is useful for a convenient determination of inhibitory activity against plant transformation.

• 社団法人日本農芸化学会の論文
• 1998-12-23

著者

• 神崎 浩

  岡山大 大学院自然科学研究科(農学系)

• KANZAKI HIROSHI

  Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• KAWAZU KAZUYOSHI

  Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Kanzaki Hiroshi

  Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Kawazu Kazuyoshi

  The Graduate School Of Natural Sci. And Technol. Dep. Of Biofunctional Chemistry Lab. Of Applied Nat

• Kawazu Kazuyoshi

  Laboratory Of Bioresources Chemistry Department Of Agricutlural Science Okayama University

• Kawazu Kazuyoshi

  Department Of Bioresources Chemistry Okayama University

• Kawazu Kazuyoshi

  Laboratory Of Bioresources Chemistry Department Of Agricultural Science Okayama University

• Kaji Hiroaki

  Department Of Immunochemistry Faculty Of Pharmaceutical Sciences Okayama University

• Kawazu K

  The Graduate School Of Natural Sci. And Technol. Dep. Of Biofunctional Chemistry Lab. Of Applied Nat

• Asano Satomi

  Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• KAGEMORI Toshihiko

  Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Kagemori Toshihiko

  Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Kagemiri Toshihiko

  Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University

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