Novel Diketopiperazine Metabolism in a Microorganism : Two-Step Hydrolysis of Cyclo(Gly-Leu)to Amino Acids and Preliminary Characterization of Cyclo(Gly-Leu)Hydrolase and Dipeptidase

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概要

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• A bacterium, strain NM 5-3, isolated from soil exhibited the highest cyclo(Gly-Leu)(CGL)-hydrolyzing activity and was identified as Agrobacterium radiobacter. The reaction products from CGL were dipeptides(Leu-Gly and Gly-Leu)and amino acids(Leu and Gly). Inhibitors for the dipeptidase of this strain did not inhibit the hydrolysis of CGL to dipeptides, indicating that two distinct enzymes, CGLase and a dipeptidase, were involved in its hydrolysis. The activities of these two enzymes were separated by anion-exchange column chromatography. The results indicated that strain NM5-3 hydrolyzed CGL via the dipeptides to the corresponding amino acids. The CGLase fraciton was found to catalyze the hydrolysis of cyclo(Gly-D-Leu), cyclo(Gly-Gly), cyclo(L-Ala-Gly), and cyclo(D-Ala-Gly). On the other hand, the dipeptidase fraction exhibited L-specific substrate specificity.

• 公益社団法人日本生物工学会の論文
• 2000-06-25

著者

• 神崎 浩

  岡山大 大学院自然科学研究科(農学系)

• KANZAKI HIROSHI

  Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• NITODA TERUHIKO

  Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• KAWAZU KAZUYOSHI

  Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Nitoda Teruhiko

  Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Nitoda Teruhiko

  Graduate School Of Natural Science And Technology Okayama University

• Kanzaki Hiroshi

  Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Kawazu Kazuyoshi

  The Graduate School Of Natural Sci. And Technol. Dep. Of Biofunctional Chemistry Lab. Of Applied Nat

• Kawazu Kazuyoshi

  Laboratory Of Bioresources Chemistry Department Of Agricutlural Science Okayama University

• Kawazu Kazuyoshi

  Department Of Bioresources Chemistry Okayama University

• Kawazu Kazuyoshi

  Laboratory Of Bioresources Chemistry Department Of Agricultural Science Okayama University

• Kaji Hiroaki

  Department Of Immunochemistry Faculty Of Pharmaceutical Sciences Okayama University

• Kawazu K

  The Graduate School Of Natural Sci. And Technol. Dep. Of Biofunctional Chemistry Lab. Of Applied Nat

• Nitoda T

  The Graduate School Of Natural Science And Technology Department Of Biofunctional Chemistry Laborato

• MIZUTA NAOKI

  Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Mizuta Naoki

  Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University

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