Association between Hepatic Triacylglycerol Accumulation Induced by Administering Orotic Acid and Enhanced Phosphatidate Phosphohydrolase Activity in Rats

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概要

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• Orotic acid is known to cause fatty liver, but it is unclear whether this is caused partly by stimulation of the enzymes for triacylglycerol(TG)synthesis.To understand the change of hepatic TG metabolism in fatty liver induced by orotic acid, we determined the liver tissue TG level and phosphatidate phosphohydrolase (PAP) activity over time in rats fed on a diet containing orotic acid (OA).A dietary lipid content of 10% was achieved by using n-6 fatty acidrich corn oil in experiment 1, and n-6 fatty acid-rich safflower oil (SO) and n-3 fatty acid-rich fish oil (FO) with the same polyunsaturated fatty acid/monounsaturated fatty acid/saturated fatty acid(P/M/S) ratio in experiment 2.In experiment 1, an increase in the hepatic TG level due to OA intake was observed from day 5 onwards, the level rising approximately 60-fold by day 10.The activity of hepatic microsomal PAP, the rate-limiting enzyme in TG synthesis, increased markedly from day 5 onwards, concurrent with the liver diacylglycerol concentration.A strong correlation (r=0.974) was observed between the hepatic TG level and microsome-bound PAP activity.In experiment 2, we investigated the effects of dietary fatty acid on OA-induced fatty liver.Compared with the n-6 fatty acid-rich vegetable oil diet, the relative increase in hepatic TG was smaller with the n-3 fatty acid-rich FO diet, and hepatic PAP activity fell markedly to the level for an OA-free diet.In addition, the hepatic TG accumulation and serum TG concentration were lower in the FO group than in the SO group.Nevertheless, because the hepatic TG level was low, it seems that the inhibition of liver PAP activity by FO possibly had a strong influence on the accumulation of TG in the liver.In conclusion, enhanced TG synthesis mediated by changes in liver PAP activity was involved in the hepatic TG accumulation induced by OA administration, this change being markedly suppressed by dietary n-3 fatty acids.

• 社団法人日本農芸化学会の論文
• 1998-03-23

著者

• Yanagita Teruyoshi

  佐賀大学 食糧科学

• Yanagita Teruyoshi

  Laboratory Of Nutrition Biochemistry Faculty Of Agriculture Saga University

• OOGAMI Kazuhiro

  Department of Applied Biological Sciences, Saga University

• Cha Jae-young

  Biohub Co. Ltd.

• Cha J‐y

  Department Of Applied Biological Sciences Saga University

• Yamamoto Kyosuke

  Department Of Internal Medicine Saga Medical School

• Yamamoto Kyosuke

  Department Of Applied Biological Sciences Saga University

• Yanagita Teruyoshi

  Wakamoto Pharmaceutical Co.

• Oogami K

  Department Of Internal Medicine Saga Medical School

• Oogami Kazuhiro

  Department Of Applied Biological Sciences Saga University

• CHA Jae-Young

  Department of Applied Biological Sciences, Saga University

• MAMEDA Yuji

  Department of Applied Biological Sciences, Saga University

• Mameda Yuji

  Department Of Applied Biological Sciences Saga University

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