Characterization of and Ethanol Hyper-production by Clostridium thermocellum I-1-B
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概要
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An ethanol hyper-producing clostridial strain, I-1-B, was isolated from Shibi hot spring, Kagoshima prefecture and identified as Clostridium thermocellum based on morphological and physiological properties. The carbohydrates used as energy sources were glucose, fructose, cellobiose, cellulose and esculin. Fermentation products were ethanol, lactate, acetate, formate, carbon dioxide, and hydrogen. The optimum, maximum, and minimum temperature for growth are about 60, 70, and 47℃, respectively. Optimum pH for growth is about 7.5, and growth occurs at starting pH between 6.0 and 9.0. I-1-B strain has strong tolerance for ethanol and hyper ethanol-productivity. Ethanol concentrations causing 50% decrease of growth yield are 27 and 16 g/liter for I-1-B and ATCC27405 of C. thermocellum, respectively. The organism was cultured on a medium containing 80 g/liter cellulose at 60℃ for 156h. The culture was fed with a vitamin mixture containing vitamin B_<12> and mineral salts solution at intervals. In this culture the organism produced 23.6 g/liter (512 mM) ethanol, 8.5 g/liter (94 mM) lactate, 2.9 g/liter (48 mM) acetate, and 0.9 g/liter (20 mM) formate. The molar ratio of ethanol to total acidic products was 3.2. The ethanol productivity of the strain I-1-B is superior to any of the wild and mutant strains of C. thermocellum so far reported.
- 社団法人日本農芸化学会の論文
- 1993-12-23
著者
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Takagi Y
Saga Univ. Saga Jpn
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Tomita Minoru
Industrial Technology Center Of Tokushima
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Takagi Y
Iwate Univ. Morioka Jpn
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OKUMA Emiko
Department of Hematology, Research Institute, International Medical Center of Japan
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Tomita Minoru
Izumi Alcohol Plant Nedo
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Sato Kanji
New Energy and Industrial Technology Development Organization (ENDO), Alcohol Production Head Office
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Yonemura Sotaro
New Energy and Industrial Technology Development Organization (ENDO), Alcohol Production Head Office
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Goto Shingo
New Energy and Industrial Technology Development Organization (ENDO), Alcohol Production Head Office
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Sekine Kenji
New Energy and Industrial Technology Development Organization (ENDO), Alcohol Production Head Office
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Okuma Emiko
New Energy and Industrial Technology Development Organization (ENDO), Alcohol Production Head Office
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Takagi Yoshio
New Energy and Industrial Technology Development Organization (ENDO), Alcohol Production Head Office
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Hon-Nami Koyu
New Energy and Industrial Technology Development Organization (ENDO), Alcohol Production Head Office
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Saiki Takashi
New Energy and Industrial Technology Development Organization (ENDO), Alcohol Production Head Office
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Takagi Yoshikazu
Technical Research Center T. Hasegawa Co. Ltd.
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Sato Kanji
New Energy And Industrial Technology Development Organization (endo) Alcohol Production Head Office
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Okuma E
Department Of Hematology Research Institute International Medical Center Of Japan
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Okuma Emiko
New Energy And Industrial Technology Development Organization (endo) Alcohol Production Head Office
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Goto S
Kinjo Univ. Nagoya Jpn
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Sekine K
Nagoya Univ. Nagoya Jpn
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Yanai Tetsuya
Technical Research Center T. Hasegawa Co. Ltd.
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Saiki Takashi
New Energy And Industrial Technology Development Organization (endo) Alcohol Production Head Office
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Hon-nami Koyu
New Energy And Industrial Technology Development Organization (endo) Alcohol Production Head Office
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Yonemura Sotaro
New Energy And Industrial Technology Development Organization (endo) Alcohol Production Head Office
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Takagi Yoshiakzu
Kawasaki Research Laboratory T. Hasegawa Co. Ltd.
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