Productivity and Some Properties of Egg Yolk Antibody (IgY) against Human Rotavirus Compared with Rabbit IgG
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概要
- 論文の詳細を見る
Productivity and some properties of anti-Human Rotavirus (HRV) hen egg yolk antibody (IgY) were compared with those of anti-HRV rabbit serum antibody (IgG). The hens immunized with HRV (Wa strain, serotype 1 and Mo strain, serotype 3) were found to continuously to lay eggs without any change in the egg laying rate and the yolk of the eggs laid over a year showed a high level of neutralization titer against HRV. The production of anti-HRV IgY by a hen (one year) was at least 15 times (anti-Wa) and 120 times (anti-Mo) more effective than those by an immunized rabbit in the neutralization titer of the antibodies. The stability of anti-HRV IgY at temperature above 70℃ and low pH 2-3 was less than that of anti-HRV rabbit IgG. The temperature corresponding to the maximum of denaturation endotherm (T_<max>) of IgY was 73.9℃ while that of rabbit IgG was 77.0℃ in the analysis by differential scanning calorimetry. This discrepancy in heat and acidic pH stability found between the two antibodies as discussed with regard to their protein structures.
- 社団法人日本農芸化学会の論文
- 1993-03-23
著者
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赤地 重宏
三重県科学技術振興センター 保健環境研究部
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Kim M
Central Research Laboratories Taiyo Kagaku Co. Ltd.
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Kim Mujo
Central Research Laboratories Taiyo Kagaku Co. Ltd.
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Kimi Mujo
Central Research Laboratories Taiyo Kagaku Co. Ltd.
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Kim Mujo
Central Research Laboratories Of Taiyo Kagaku Co. Ltd.
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Kim M
Taiyo Kagaku Co. Ltd. Mie Jpn
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YAMAMOTO Takehiko
Department of Biotechnology, Fukuyama University
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Yamamoto T
Novelty Materials Research Institute Hannan R&d Center Fuji Oil Co.ltd.
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Hatta Hajime
Central Research Laboratories, Taiyo Kagaku Co., Ltd.
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Tsuda Ken
Central Research Laboratories, Taiyo Kagaku Co., Ltd.
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Akachi Sigemitu
Central Research Laboratories, Taiyo Kagaku Co., Ltd.
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Kim M
Department Of Nutrition And Food Science Ochanomizu University
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Hatta H
Kyoto Women's Univ. Kyoto Jpn
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Hatta Hajime
Central Research Laboratories Taiyo Kagaku Co. Ltd.
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Yamamoto T
Department Of Biotechnology Fukuyama University
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Yamamoto Takehiko
Department Of Biology Faculty Of Science Osaka City University
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Tsuda K
Central Research Laboratories Taiyo Kagaku Co. Ltd.
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Akachi Sigemitu
Central Research Laboratories Taiyo Kagaku Co. Ltd.
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