Characterization of a Class III Chitinase from Vitis vinifera cv. Koshu(GENETICS,MOLECULAR BIOLOGY,AND GENE ENGINEERING)
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概要
- 論文の詳細を見る
A chitinase gene (Chi3K) was cloned from the genomic DNA of Vitis vinifera cv. Koshu. The structural gene comprised 891 bp without introns and encoded 297 amino acids. The Chi3K product showed high similarity to the class III chitinase of V. vinifera cv. Pinot noir. Chi3K was expressed using a bacterial expression vector for purification and enzymatic characterization of its gene product. The recombinant chitinase exhibited hydrolytic activity toward glycol chitin and its optimum pH was 4.0. It also inhibited the growth of Botrytis cinerea, which causes grey mold disease in grapes.
- 社団法人日本生物工学会の論文
- 2003-06-25
著者
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Ano Akihiko
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Yokotsuka K
Univ. Yamanashi Kofu
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Yokotsuka Koki
The Institute Of Enology And Viticulture Yamanashi University
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Yokotsuka Koki
Interdisciplinary Graduate School Of Medicine And Engineering & The Institute Of Enology And Vit
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Okuda Tohru
The Institute Of Low Temperature Science Hokkaido University
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Okuda Tohru
The Institute Of Enology And Viticulture Yamanashi University
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Yokotsuka Koki
The Institute Of Enology And Viticulture
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TAKAYANAGI TSUTOMU
The Institute of Enology and Viticulture, Yamanashi University
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UCHIBORI TAKAYUKI
The Institute of Enology and Viticulture, Yamanashi University
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Uchibori Takayuki
The Institute Of Enology And Viticulture Yamanashi University
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Takayanagi T
Interdisciplinary Graduate School Of Medicine And Engineering & The Institute Of Enology And Vit
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Takayanagi Tsutomu
The Institute Of Enology And Viticulture Yamanashi University
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Yokotsuka Koki
The Institute Of Ecology And Viticulture Yamanashi University
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