Purification and Properties of Invertase from Muscat Bailey A Grapes
スポンサーリンク
概要
- 論文の詳細を見る
A grape invertase was purified from Muscat Bailey A juice by salting out with ammonium sulfate and successive chromatographies on Sephadex G-100 and Con A-agarose to a homogeneous state as confirmed by polyacrylamide gel electrophoresis. The molecular weight of the purified enzyme was 72 kDa in gel filtration chromatography. However, SDS polyacrylamide gel electrophoresis revealed three bands with molecular weights of 56 kDa, 25 kDa and 24 kDa. Affinity staining of Western blots with lectins in dicated that the enzyme was a glycoprotein. The optimum pH for the enzyme reaction was 3.5 and the optimum temperature 80℃. The enzyme was stable from pH 2.7 to 6.4,and up to 80℃. The transfructosylation reaction could not be observed. The K_m value of this enzyme for sucrose was 4.4 mM at pH 4.0,but as the pH of the reaction mixture increased, the K_m value decreased sharply. From the dependence of the V_<max> and K_m values on pH, the ionization constant (pK_e) of one of the two essential ionizable groups of the free enzyme was determined to be 2.7,suggesting that this essential ionizable group was a carboxyl.
- 公益社団法人日本生物工学会の論文
- 1994-10-25
著者
-
Porntaveewat Wimolsiri
The Institute Of Enology And Viticulture Yamanashi University
-
Takayanagi Tsutomu
The Institute Of Enology And Viticulture Yamanashi University
-
Yokotsuka Koki
The Institute Of Ecology And Viticulture Yamanashi University
関連論文
- Preventive Effects of the Plant Isoflavones, Daidzin and Genistin, on Bone Loss in Ovariectomized Rats Fed a Calcium-Deficient Diet
- Analysis of Lactic Acid Bacterial Flora during Miso Fermentation
- Changes in Isoflavone Compositions of Soybean during Soaking in Water
- Changes in Isoflavone Compositions of Soybean Foods during Cooking Process
- Turbidity Formation Caused by Interaction of Must Proteins with Wine Tannins : Chemical Studies on Coloring and Flavoring Substances in Japanese Grapes and Wines (XVII).
- Characterization of a Class III Chitinase from Vitis vinifera cv. Koshu(GENETICS,MOLECULAR BIOLOGY,AND GENE ENGINEERING)
- Restriction Fragment Length Polymorphism Analysis of 16S rRNA Genes in Lactic Acid Bacteria Isolated from Red Wine
- Isolation and Structure of Antimicrobial Substances from Paprika Seeds
- Comparison of Composition of Wines Made Using Liquid Starters of Saccharomyces cerevisiae W3 and OC2 and Active Dry Yeast Forms of the Same Strains
- An Improved Method for Fluorometric Amino Acid Analysis Including Proline and Hydroxyproline : Chemical Studies on Coloring and Flavoring Substances in Japanese Grapes and Wines (XIV)
- Contents of Resveratrol, Piceid, and Their Isomers in Commercially Available Wines Made from Grapes Cultivated in Japan
- A New Method for Fluorometric Measurement of Proline in Grapes and Wines
- Purification and Properties of Invertase from Muscat Bailey A Grapes
- Inhibitory Effect of Paprika Seed Extract on the Growth of Yeast.
- Growth Inhibition of Film-Forming Yeasts During Production of Ume-Zuke (Pickled Japanese Apricots) by an Antimicrobial Fraction Extracted from Paprika Seeds.
- Changes in Color Parameters during Fermentation and Storage of Red Wine Using Thai Roselle under Different pHs and SO2 Concentrations.