Characterization of Oligo(dT)-Binding Proteins from Seedlings of Winter Wheat
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概要
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Oligo(dT)-binding proteins of winter wheat(Triticum aestivum L.)seedlings were separated into two fractions by chromatography on oligo(dT)-cellulose;one fraction was eluted sequentially with 10 mM Tris-HCl(D fraction)and the other was eluted with the same buffer supplemented with 500g L^<-1> formamide(formamide fraction). The proteins associated with the oligo(dT)-cellulose were characterized by SDS-polyacrylamide gel electrophoresis(PAGE)and, out of the more than 17 polypeptides present in the fractions, a set of proteins common to both the D and formamide fractions had an apparent molecular mass of 55 kDa. Two other sets of the isoform proteins with a molecular mass of 35 and 29 kDa, respectively, were present in the formamide fraction. After growth under snow for three months, an apparent increase in the level of polypeptide with a molecular mass of about 80-kDa was detected. Studies with a cell-free translation system showed that the addition of each fraction resulted in a 1.7- to 3.8-fold stimulation of the rate of incorporation of L-[^<35>S]-methionine into acid-insoluble material relative to the control rate(without addition). The nature of the products of translation and the efficiency of their synthesis were influenced by the presence of added oligo(dT)-binding proteins. It is suggested that oligo(dT)-binding proteins of winter wheat seedlings are involved in the regulation of the translational activity of poly(A)^+ RNA.
- 社団法人日本土壌肥料学会の論文
著者
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SAGISAKA Shonosuke
The Institute of Low Temperature Science, Hokkaido University
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Sagisaka Shonosuke
The Institute Of Lou Temperature Science Hokkaido University
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Okuda Tohru
The Institute Of Low Temperature Science Hokkaido University
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Okuda Tohru
The Institute Of Enology And Viticulture Yamanashi University
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Yamanaka Akira
The Institute Of Low Temperature Science Hokkaido University
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Sagisaka S
The Institute Of Low Temperature Science Hokkaido University
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Matsuda Yusuke
The Institute of Low Temperature Science, Hokkaido University
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Matsuda Y
Iwate Univ. Morioka Jpn
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