THE CHARACTERISTICS OF LUTEINIZlNG HORMONE AND ITS β-SUBUNIT SECRETION IN DISPERSED RAT ANTERIOR PITUITARY CELLS
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概要
- 論文の詳細を見る
ラット下垂体前葉遊離培養細胞のカラム灌流法を用いてLHおよびβサブユニットの分泌動態を検討し,以下の結果を得た.1) LH-RHの間歇的投与では,10^<-10>より10^<-6>MでLHおよびLH-β分泌に容量反応性を認めた.2) 10^<-8>M LH-RHの間歇的投与では,ほぼ一定したLHおよびLH-β分泌が認められ,self-priming effect, desensitizationとも明らかでなかった.3) 10^<-8>M LH-RHの持続的投与では,投与開始時に一過性の大きなLHおよびLH-β分泌が見られたが,時間とともに分泌は減少しdesensitizationが認められた.以上より,Cytodex beadsを応用したカラム灌流法はLHのみならずサブユニット分泌を経時的に検討する上でも優れた方法であると思われた.またLH-βはLHと分泌パターンが類似し,LH-RH刺激によりLHとともに下垂体前葉細胞より直接分泌される可能性が示唆された.
- 社団法人日本産科婦人科学会の論文
- 1987-12-01
著者
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Seki Takashi
Department ot Pediatrics, Nippon Medical School
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Makino Tsunehisa
Departments Of Obstetrics And Gynecology Tokai University
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Makino Tsunehisa
慶応義塾大学 産婦人科
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TAKAHASHI Mamoru
Department of Surgery, Division of Frontier Medical Science, Graduate School of Biochemical Sciences
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IIZUKA Rihachi
Department of Obstetrics and Gynecology School of Medicine, Keio University
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Seki Takashi
Department Ot Pediatrics Nippon Medical School
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Iizuka R
Department Of Obstetrics And Gynecology School Of Medicine. Keio Uneiversity
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Iizuka Rihachi
Department Of Obstetrics And Gynecology School Of Medicine Keio University
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Tanimoto Satoshi
Department of Electronic Engineering, Tokyo University of Agriculture and Techonlogy
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Takahashi Mamoru
Department Of Surgery Division Of Frontier Medical Science Graduate School Of Biochemical Sciences H
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Tanimoto Satoshi
Department Of Obstetrics And Gynecology School Of Medicine. Keio Uneiversity
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IIDA Toshihiko
Department of Obstetrics and Gynecology, School of Medicine. Keio Uneiversity
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Iida Toshihiko
Department Of Obstetrics And Gynecology School Of Medicine. Keio Uneiversity
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Makino Tsunehisa
Department Of Obstetrics And Gynecology Center For Growth And Reproductive Medicine Tokai University
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Makino Tsunehisa
Department Of Obstetrics And Gynecology School Of Medicine Keio University
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Takahashi Mamoru
Department Of Industrial Chemistry Faculty Of Engineering University Of Tokyo
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Makino T
Keio Univ. Tokyo Jpn
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Makino Tsunehisa
Department Of Obstetrics And Gynecology
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Takahashi Mamoru
Department Of Agricultural Chemistry Utsunomiya University
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Takahashi Mamoru
Department Of Obstetrics And Gynecology School Of Medicine. Keio Uneiversity
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Makino Tsunehisa
Department Of Obstetrics & Gynecology School Of Medicine Tokai University
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Seki Takashi
Department Of Obstetrics And Gynecology School Of Medicine. Keio Uneiversity
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Seki Takashi
Department Of Cardiology Sendai Medical Center
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MAKINO TSUNEHISA
Department of Gynecology and Obstetrics, School of Medicine, Keio University
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IIZUKA RIHACHI
Department of Obstetrics & Gynecology, School of Medicine, Keio University
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