Luke 法抽出, ゲル浸透クロマトグラフィー, ガスクロマトグラフィーによる多成分残留農薬分析法の検討

概要

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We studied multi residue method of pesticides using Luke Method extraction, gel permeation chromatography (GPC) and dual column GC. A sample was extracted with acetone and filtered. Aliquot of the extracts was extracted with dichloromethane-petroleum ether (1 : 1) without evaporation of acetone. The extract was dehydrated, evaporated in vacuo, dissolved in dichloromethane-cyclohexane (1 : 1) and cleaned up by GPC using Bio Beads S-X3 and mobile phase dichloromethane-cyclohexane (1 : 1). The eluate fraction of pesticides was evaporated in vacuo and dissolved in acetone. The sample was determined with a dual column GC (column DB-1 and DB-1701, or DB-210 and DB-1) equipped with NPD and FPD. Silica gel column fractionation was effective for furthermore clean-up. Recoveries of 65 organic phosphate, carbamate and nitrogen containing pesticides from fortified tomato, lettuce and strawberry ranged 53-122%, except propamocarb, acephate, methamidophos and aldicarb. This multi residue method is fast and convenient to determine pesticide residues in crops.
日本農薬学会の論文
1995-05-20