生薬およびその製剤の理化学的品質評価に関する研究(第1報)葛根および葛根湯の分析

概要

論文の詳細を見る
Daidzein, daidzin and puerarin in MeOH extracts of Pueraria Radix and Species Puerariae were analyzed using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). For standards, daidzein, daidzin and puerarin were isolated from Pueraria Radix. For qualitative analysis, TLC was carried out using silica gel plate and CHCl_3-MeOH-H_2O (65 : 35 : 10,lower layer). Daidzein and puerarin were detected by exposing to ultraviolet (UV) light, and daidzin was detected by spraying p-anisaldehyde-H_2SO_4 solution. For determination, HPLC was carried out using Shodex ODS as separation column and CH_3CN-H_2O (15 : 85) as mobile phase. Daidzein and daidzin were determined using UV detector at 254 nm. Puerarin was determined using fluorescence detector at λ_<EX> 254nm. Samples were extracted with MeOH. The extracts were passed through Sep Pak C_<18> cartridge and applied to TLC and HPLC. Recoveries of these constituents from model preparation were as follows ; daidzein : 86%, daidzin : 96% and puerarin : 99%, respectively. These methods were considered to be useful for one of evaluations of commercial Pueraria Radix and Species Puerariae.
公益社団法人日本薬学会の論文
1984-01-25