BMP誘導異所性硬組織界面の骨芽細胞分化と骨基質蛋白遺伝子の発現 : In Situ Hybridization法による検討

概要

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Osteopontin(Osp), osteonectin(Osn),osteocalcin(Osc) gene in new formational bone induced by bone morphogenetic protein(BMP) and tooth germ were examined in this study. BMP-carrier(collagen I) composites were implanted in subcutaneous tissue of the back of rats. At 1,2,3 weeks after implanted, all specimens were immersed in 4% paraformaldehyde and processed for routine light microscopy with or without decalcification. For in situ hybridization, we use single strand RNA probes by DIG RNA Labeling Kit, and stained with Nucleic Acid Detection Kit(Boehringer Mannheim). Osp and Osn signals both appears on the osteoblastic or fibroblastic cells at 1 week after implantation. Osc signal appears on the osteoblasts at 2 weeks after implantation. Osc signal increase on the osteoblasts around mineralized trabecular bone at 3 weeks after implantation. These results suggest that distribution of these bone matrix protein gene was associated with growth and mineralization of bone formation.
硬組織再生生物学会の論文
1994-03-25