ブタ顎関節関節円板、後部結合組織、下顎頭線維層におけるプロテオグリカンの比較検討

概要

論文の詳細を見る
The purpose of this study was to characterize the biochemical and immunological properties of the temporomandibular joint (TMJ) disk, retrodiskal tissue and superficial layer of condyle proteoglycans (PGs) in porcine. PGs were extracted with 4M guanidine chloride from the porcine TMJ disk, retrodiskal tissue and superficia1 1ayer of condyle and partial purified by ion-exchange chromatography. The PG fragments were digested with pronase E and assaied by cellulose acetate membrane electrophoresis. Glycosaminoglycans (GAGs) were digested with chondroitinase ABC or AC2 and core proteins were assaied by western-blotting. A high molecular weight PG resembled to the cartilage PG, and two low moleculaer weight PGs resembled to Biglycan (PG-1) and Decorin (PG-2). Compared with the proportion of PGs at these tissues, the proportion of PGs at TMJ disk resembled to that of retrodiskal tissue, but the proportion of PGs at superficial layer of condyle was dirrerent from the two PGs. Because TMJ disk had higher proportion of Dermatan sulrate/Chondroitin sulfate than those in retrodiskal tissue. TMJ disk is seemed to have more strong tensile strength resistibility than retrodiskal tissus.
硬組織再生生物学会の論文
1995-11-10