エナメル質の基質代謝と石灰化形成

概要

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The purpose of this study was to investigate the mineralization mechanism in developing enamel using pH staining. Unerupted bovine teeth were used for the expriment. The activity of a proteolytic enzyme was evaluated against enamel protein obtainedfrom bovine enamel. Crystals in developing enamel, which were classlfied into neutral zone 1 and 2, acid zone 1 and 2, were investigated using infrared spectroscopy, thermal analysis, and power X-ray diffractometry. Proteolytic enzyme showed the highest activity at approximately pH6.0, which was in accordance with the pH at the acidic zone. Most of the organic components of the enamel decomposed at 580℃, except for the carbonate and hydorogen phosphate groups included in the apatite crystals. In the developing enameL (Ca+Mg)/P molar ratio was lower than that of the stoichometric hydroxyapatite. Furthermore, carbonate amount, which were considered to replace the OH and PO4 sites in the apatite crystal, was higher than those in the matured enamel. The a-axis dimension of the apatite crystals in the secretory stage enamel (Neutral zone 1) was confirmed to be greater than that in acidic zone 2 and matured enamel. These results indicated that enamel mineralization was followed by decarbonation in carbonate apatite.
硬組織再生生物学会の論文