顎関節成分の生理機能と役割

概要

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The purpose of this study was to characterize the biochemioal and immunological properties of the temporomandibular joint (TMJ) disk, retrodiskal tissue and superficial layer of condyle proteoglycans (PGs). PGs were extracted with 4M guanidine chloride from the porcine TMJ disk, retrodiskal tissue and superficial layer of contyle, and partial punfied by ion-exchange chromatography. The PGs fragments were digested with pronase E and assayed by cellalose acetate membrane electrophoresis. Glycosaminoglycans (GAGs) were digested with chondroitinase ABC or AC2, and core proteins were assayed by western-blotting. The high molecular weight PG resembled the cartilage PG, and two low molecular weight PG s resembled Biglycan (PG-1) and Decorin(PG-2). Compared with these PGs, TMJ disk PGS and retrodiskol tissue PGS resembled the tendon of Achilles PGs, but the superficial layer of condyle PGS was different from others. TMJ disk PGS had higher Dermatan sulfate/Chondroitin sulfate than retrodiskal tissue PGs, that TMJ disk had more strong tensile strength resistibility than retrodiskal tissue.These results suggest that this difference is connected with the origin of temporomandibular disorders (TMD).
硬組織再生生物学会の論文