ウシ骨由来粗精製BMPの異所性骨誘導に関する基礎的研究

概要

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Fresh bovine metatarsal bones were cleaned of periosteum and marrow, pulverized,demineralized in HCI, and extracted with 4 M guanidine HCl/0.05 M Tris-HCI (pH 7.4) containingprotease inhibitors. The extract was filtrated and concentrated to limit the molecular weight ofproteins to 10-100 kDa. This limited molecular weight extract was dialyzed against distilledwater, and the water insoluble precipitation was collected by centrifugation. The BMP extract atthis stage was called crude BMP. Type I collagen was extracted and purified from the guanidineinsoluble collagenous residues of the bovine demineralized bone matrix (IBM). IBM was digestedwith pepsin to reduce the antigenicity. Crude BMP was combined with the bovine bone type Icollagen as a carrier. The BMP/carrier mixtures were implanted in the dorsal subcutaneoustissues of 4-week old male rats. The implants were removed at 1, 2 and 3 weeks afterimplantation, and the induced ectopic hard tissues were observed by routine histology. The type I collagen did not cause a remarkable immunogenic reaction. The results suggest that the mixtureof the molecular weight limited BMP and bovine bone type I collagen is a superior biomaterialfor the induction of hard tissue.
硬組織再生生物学会の論文
1998-10-04