Functional enucleation of mouse metaphase II oocytes with etoposide
スポンサーリンク
概要
- 論文の詳細を見る
Mouse metaphase II (M II) oocytes were exposed to 50 μg/ml etoposide (ETO) before and after parthenogenetic activation with 7% ethanol and they were washed with 0.75 M sucrose. The ETO treated parthenogenetically activated oocytes were cultured or fused to single blastomeres of late 2-cell stage mouse embryo to test their ability to support development in vitro. In parallel untreated parthenogenetically activated oocytes were cultured to serve as control. None of ETO treated oocytes developed beyond the 2-cell stage, whereas 4% of the reconstituted embryos and 35% of control developed to blastocysts. It is concluded that mouse M II oocytes can be functionally enucleated by ETO treatment and can be used for nuclear transfer experiments.
- 北海道大学の論文
- 1998-02-27
著者
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KATAGIRI Seiji
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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TAKAHASHI Yoshiyuki
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Takahashi Yoshiyuki
北海道大学 獣医学研究科繁殖学教室
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Takahashi Yoshiyuki
Graduate School Of Veterinary Medicine Hokkaido University
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Katagiri Seiji
Lab. Of Theriogenology Dep. Of Veterinary Clinical Sciences Graduate School Of Veterinary Medicine H
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Katagiri Seiji
Laboratory Of Theriogenology Department Of Clinical Sciences Graduate School Of Veterinary Medicine
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Takahashi Y
Laboratory Of Theriogenology Department Of Clinical Sciences Graduate School Of Veterinary Medicine
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Takahashi Yoshiyuki
Lab. Of Theriogenology Dep. Of Veterinary Clinical Sciences Graduate School Of Veterinary Medicine H
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KANAGAWA Hiroshi
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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ELSHEIKH Adil
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Kanagawa H
Hokkaido Univ. Sapporo Jpn
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Elsheikh Adil
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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Takahashi Yoshiyuki
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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Takahashi Y
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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Kanagawa Hiroshi
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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Kanagawa Hiroshi
Laboratory of Theriogenology, Department of Veterinary, Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University
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