Effect of activation treatments of recipient oocytes on subsequent development of bovine nuclear transfer embryos
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概要
- 論文の詳細を見る
Effects of recipient oocyte activation methods on the development of nuclear transfer (NT) embryos were investigated. In Exp. 1, cell-cycle phase of serum-starved bovine cumulus cells was examined by flow cytometry. Majority (95.5%) of medium-sized (16-20 μm) cells that made up 56% of total cells was at the G_0/G_1 phase. NT embryos were constructed by electric fusion with the medium-sized serum-starved cumulus cells and bovine oocytes of 3 different preparations: enucleated oocytes treated with calcium ionophore A 23187 for 5 min and cycloheximide for 5 hr (A 23187/CHX), those treated with ethanol for 7 min and cycloheximide for 2 hr (ethanol CHX) and those without treatment. In Exp. 2 and 3, developmental competence of NT embryos constructed with A 23187/CHX- and ethanol/HX-treated oocytes was compared to that of NT embryos constructed with non-treated oocytes, respectively. Further, nuclear behavior in 3 different NT embryos was examined in Exp. 4. Within 1 hr after fusion, majority of the NT embryos constructed with non-treated oocytes showed condensed chromosome. Three hours after fusion, about 50% of NT embryos constructed with non-treated or ethanol/CHX-treated oocytes showed a single pronucleus-like structure. NT embryos constructed with ethanol/CHX-treated oocytes showed similar rates of fusion, cleavage and blastocyst formation to those of the non-treated oocytes. In contrast, NT embryos constructed with A 23187/CHX-treated oocytes did not show any pronucleus-like structure and showed lower cleavage rate and no development to blastocysts. The results indicate that ethanol/CHX-treated oocytes could support development of somatic cell NT embryos to the blastocyst stage at a similar rate to that of non-treated oocytes.
- 北海道大学の論文
- 2003-02-28
著者
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KATAGIRI Seiji
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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TAKAHASHI Yoshiyuki
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Takahashi Yoshiyuki
北海道大学 獣医学研究科繁殖学教室
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Atabay Edwin
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Atabay Edwin
北海道大学 獣医学研究科診断治療学講座繁殖学
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Takahashi Yoshiyuki
Graduate School Of Veterinary Medicine Hokkaido University
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Katagiri Seiji
Lab. Of Theriogenology Dep. Of Veterinary Clinical Sciences Graduate School Of Veterinary Medicine H
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Katagiri Seiji
Laboratory Of Theriogenology Department Of Clinical Sciences Graduate School Of Veterinary Medicine
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Atabay Edwin
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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NAGANO Masashi
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Takahashi Y
Laboratory Of Theriogenology Department Of Veterinary Clinical Science Graduate School Of Veterinary
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Takahashi Yoshiyuki
Lab. Of Theriogenology Dep. Of Veterinary Clinical Sciences Graduate School Of Veterinary Medicine H
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Takahashi Yoshiyuki
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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Takahashi Y
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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