Effects of cycloheximide treatment on in-vitro development of porcine parthenotes and somatic cell nuclear transfer embryos
スポンサーリンク
概要
- 論文の詳細を見る
This study aimed to verify the beneficial effect of cycloheximide (CHX) treatment on the development of porcine somatic cell nuclear transfer (NT) embryos, which were constructed with enucleated oocytes and cumulus cells by using a single direct current (DC) pulse. In the first experiment, a single DC pulse applied to the induction of fusion and activation of NT embryos gave a high fusion rate. However, cleavage and subsequent development of fused couplets (NT embryos) to the blastocyst stage were poor. Experiment 2 was conducted to determine whether CHX treatment could enhance metaphase II (M II) oocyte activation and improve the subsequent parthenogenetic development. After giving the DC pulse and incubation with or without CHX, M II oocytes incubated with CHX showed higher cleavage and development to blastocysts compared with those incubated without CHX (P <0.05). Experiment 3 was carried out to verify the beneficial effect of CHX on the development of NT embryos. The NT embryos treated with the DC pulse and CHX treatment showed higher cleavage and subsequent development compared with those treated with the DC pulse alone (P <0.05). The present study demonstrates that CHX treatment enhances the electrical stimulus-induced activation of oocytes and NT embryos, and improves the subsequent development of parthenotes and NT embryos. The results indicate that protein synthesis inhibition treatment required for the induction of oocyte activation promotes the development of NT embryos.
- 北海道大学の論文
- 2003-02-28
著者
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TAKAHASHI Yoshiyuki
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Suzuki Masatsugu
北海道大学 獣医学研究科野生動物学
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Takahashi Yoshiyuki
北海道大学 獣医学研究科繁殖学教室
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Takahashi Yoshiyuki
Graduate School Of Veterinary Medicine Hokkaido University
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Diaz Mario
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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Takahashi Y
Laboratory Of Theriogenology Department Of Veterinary Clinical Science Graduate School Of Veterinary
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Takahashi Yoshiyuki
Lab. Of Theriogenology Dep. Of Veterinary Clinical Sciences Graduate School Of Veterinary Medicine H
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Suzuki Masatsugu
Graduate School Of Veterinary Medicine Hokkaido University
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Suzuki Masatsugu
Laboratory Of Wildlife Biology Department Of Environmental Veterinary Science Graduate School Of Vet
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Suzuki Masatsugu
Laboratory Of Wildlife Biology Department Of Environmental Veterinary Sciences The Graduate School O
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Sasaki M
Laboratory Of Veterinary Anatomy Department Of Basic Veterinary Medicine Obihiro University Of Agric
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Suzuki Masami
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Kagawa Masumi
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Ikeda Koji
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Kagawa Masumi
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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Takahashi Yoshiyuki
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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Takahashi Y
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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Ikeda K
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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Ikeda Koji
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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Sasaki Motoki
Laboratory Of Veterinary Anatomy Department Of Basic Veterinary Medicine Obihiro University Of Agric
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Suzuki Masami
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences School Of Veterinary Medicin
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Ikeda Koji
Laboratory Of Hygienic Chemistry And Molecular Toxicology Gifu Pharmaceutical University
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