Cryopreservation of the Semen Collected by Electroejaculation from the Hokkaido Brown Bear(Ursus arctos yesoensis).
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概要
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Four adult Hokkaido brown bears were used as semen donors, and semen characteristics were examined before freezing and after thawing. A total of 10 electroejaculates were diluted with Tris-egg yolk extender and cooled to 4°C over 90 min. Spermatozoa were equilibrated with 4.7% glycerol for 80 min. Semen packed in 0.25 ml plastic straws were frozen with liquid nitrogen vapor. Percentages (mean ± SD) of motile and live sperm were 96 ± 2 and 86.5 ± 7.2% before freezing, and 43 ± 5 and 67.4 ± 3.9% after thawing, respectively. Although the number of progressively motile sperm after thawing varied among samples (1.8 ± 1.2 × 108 cells/ejaculate), frozen semen in the present study might serve for artificial insemination.
- 公益社団法人 日本獣医学会の論文
著者
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Katagiri Seiji
Laboratory Of Theriogenology Department Of Clinical Sciences Graduate School Of Veterinary Medicine
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Ishikawa Akiko
Laboratory Of Theriogenology Department Of Clinical Sciences Graduate School Of Veterinary Medicine
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SAKAMOTO Hideyuki
Noboribetsu Bear Park
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Matsui Motozumi
Laboratory Of Theriogenology Department Of Veterinary Clinical Science Graduate School Of Veterinary
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Takahashi Yoshiyuki
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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