ウシ胚培養液作製における紫外線照射装置および限外濾過膜を組み込んだ超純水装置の有効性
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概要
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The efficacy of a water purification system including a high-intensity 185 nm ultra violet lamp with ultrafilter (UV-UF) for eliminating endotoxins was examined. The activity of endotoxins was below the detectable level in UV-UF water. In contrast, ultra-purified water from a system without a UV lamp (UP) showed a high level of endotoxins, despite water resistivity of more than 18 MΩ·cm. Bovine IVM/IVF zygotes were cultured for 174 h in protein-free media prepared with UV-UF and UP water. The developmental rate to blastocysts at 150 h was significantly higher in the medium prepared with UV-UF water. The results demonstrate that the UV-UF system is beneficial for eliminating endotoxins from ultra-purified water, and for preparing protein-free media for culture of bovine embryos.
- 日本繁殖生物学会の論文
- 1999-06-01
著者
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KATAGIRI Seiji
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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TAKAHASHI Yoshiyuki
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Takahashi Yoshiyuki
北海道大学 獣医学研究科繁殖学教室
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Takahashi Yoshiyuki
Graduate School Of Veterinary Medicine Hokkaido University
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Katagiri Seiji
Lab. Of Theriogenology Dep. Of Veterinary Clinical Sciences Graduate School Of Veterinary Medicine H
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永野 昌志
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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高橋 芳幸
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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片桐 成二
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Katagiri Seiji
Laboratory Of Theriogenology Department Of Clinical Sciences Graduate School Of Veterinary Medicine
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NAGANO Masashi
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Takahashi Y
Laboratory Of Theriogenology Department Of Veterinary Clinical Science Graduate School Of Veterinary
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Takahashi Yoshiyuki
Lab. Of Theriogenology Dep. Of Veterinary Clinical Sciences Graduate School Of Veterinary Medicine H
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Takahashi Yoshiyuki
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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Takahashi Y
Laboratory Of Theriogenology Department Of Veterinary Clinical Sciences Graduate School Of Veterinar
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