ウシ胚培養液作製における紫外線照射装置および限外濾過膜を組み込んだ超純水装置の有効性

概要

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The efficacy of a water purification system including a high-intensity 185 nm ultra violet lamp with ultrafilter (UV-UF) for eliminating endotoxins was examined. The activity of endotoxins was below the detectable level in UV-UF water. In contrast, ultra-purified water from a system without a UV lamp (UP) showed a high level of endotoxins, despite water resistivity of more than 18 MΩ·cm. Bovine IVM/IVF zygotes were cultured for 174 h in protein-free media prepared with UV-UF and UP water. The developmental rate to blastocysts at 150 h was significantly higher in the medium prepared with UV-UF water. The results demonstrate that the UV-UF system is beneficial for eliminating endotoxins from ultra-purified water, and for preparing protein-free media for culture of bovine embryos.