Effect of Butylated Hydroxytoluene on Dog Sperm Longevity in Chilling Storage and Cryopreservation
スポンサーリンク
概要
- 論文の詳細を見る
This study evaluated the effect of butylated hydroxytoluene (BHT), a lipid-soluble antioxidant, on dog sperm in chilling storage and cryopreservation. In Experiment 1, 0.2, 0.4, 0.8 and 1.6 mM BHT were supplemented to egg yolk Tris extender (EYT) and sperm were stored at 4℃ for 96 hr. Sperm motility, viability, acrosomal integrity and morphological abnormality in the BHT treatment groups were not different from control (0 mM BHT). In Experiment 2, the effect of BHT in EYT containing 0.75% Equex STM paste and 5% glycerol on survivability of cryopreserved sperm was examined after culture at 39℃ for 3 hr. Sperm motility, viability and acrosomal integrity in 0.2 to 0.8 mM BHT treatment groups were not different from control. However, sperm motility, viability and acrosomal integrity decreased when 1.6 mM BHT was added to the extender (P<0.05). In conclusion, it is clarified that supplementation of 0.2 to 0.8 mM BHT in the extender did not affect characteristics of dog sperm in chilling storage and cryopreservation. Supplementation of 1.6 mM BHT did not affect characteristics of chilled sperm but impaired longevity of cryopreserved sperm in dog.
著者
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NAGANO Masashi
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterin
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Hishinuma Mitsugu
Department Of Animal Science Faculty Of Agriculture Tottori University
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YAMASHITA Yasuhisa
Laboratory of Animal Physiology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima
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YAMASHITA Yasuhisa
Laboratory of Physiology, Department of Life Sciences, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima
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NAGANO Masashi
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University
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HIGAKI Shogo
Department of Theriogenology, School of Veterinary Medicine, Faculty of Agriculture, Tottori University
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SAHASHI Yu
United Graduate School of Veterinary Science, Yamaguchi University
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OTSUKI Tsubasa
Department of Theriogenology, School of Veterinary Medicine, Faculty of Agriculture, Tottori University
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