Enhanced Freezability of Goat Spermatozoa Collected into Tubes Containing Extender Supplemented with Bovine Serum Albumin (BSA)

スポンサーリンク

概要

• 論文の詳細を見る

• The purpose of this study was to investigate the effects of semen collection into tubes containing extender supplemented with BSA on the cryosurvival of goat spermatozoa. Semen was collected from two goats into empty tubes or tubes containing 10 ml extender supplemented with 0, 0.1, 1, or 5% BSA, and the washed spermatozoa were frozen as pellets in egg yolk-trehalose extender with the addition of 0.04% SDS and 4% glycerol. Sperm motion parameters were evaluated after post-thawing and during a thermal resistance test. The acrosome status of frozen-thawed spermatozoa was also observed using FITC-PNA staining. In frozen semen that was collected into tubes containing extender supplemented with 5% BSA, the post-thawed spermatozoa exhibited a significant improvement in motion parameters and maintained high motility throughout incubation and acrosome integrity, as compared with semen collected into tubes containing extender supplemented with lower concentrations of BSA. In conclusion, semen collection into tubes with a large volume of extender containing high concentrations of BSA dramatically improves the motility and acrosome integrity of frozen-thawed spermatozoa. This suggests that the in vitro functional freezability of spermatozoa is abruptly modified by reducing contact with seminal plasma and by flash contact with BSA at ejaculation.

• 日本繁殖生物学会の論文

著者

• Terada Takato

  Laboratory Of Animal Reproduction Graduate School Of Biosphere Sciences

• YAMASHIRO Hideaki

  Laboratory of Animal Reproduction, Graduate School of Biosphere Sciences, Hiroshima University

• KUMAMOTO Kanako

  Laboratory of Animal Reproduction, Graduate School of Biosphere Sciences, Hiroshima University

• WANG HaiFeng

  Laboratory of Animal Reproduction, Graduate School of Biosphere Sciences, Hiroshima University

• YAMASHITA Yasuhisa

  Laboratory of Animal Physiology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima

関連論文

• 希釈液を入れた試験管に採精することにより精漿成分との接触を抑制されたヤギ射出精子の性状について
• Effect of Butylated Hydroxytoluene on Dog Sperm Longevity in Chilling Storage and Cryopreservation
• ブタ卵子の体外成熟培養時において性腺刺激ホルモンに刺激された卵丘細胞のプロジェステロン生産にはCa〔2+〕-PKC系とcAMP-PKA系が共に必要である
• The Release of EGF Domain from EGF-like Factors by a Specific Cleavage Enzyme Activates the EGFR-MAPK3/1 Pathway in Both Granulosa Cells and Cumulus Cells During the Ovulation Process
• The Release of EGF Domain from EGF-like Factors by a Specific Cleavage Enzyme Activates the EGFR-MAPK3/1 Pathway in Both Granulosa Cells and Cumulus Cells During the Ovulation Process
• Enhanced Freezability of Goat Spermatozoa Collected into Tubes Containing Extender Supplemented with Bovine Serum Albumin (BSA)

スポンサーリンク