Immunohistochemical Localization of Steroidogenic Enzymes in the Testis of the Sika Deer (Cervus nippon) During Developmental and Seasonal Changes
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概要
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Testicular steroidogenesis and spermatogenesis during developmental and seasonal changes were investigated in male sika deer (Cervus nippon), a short-day seasonal breeder, to clarify the physiological mechanisms for reproductive function. The immunohistochemical localization of steroidogenic enzymes (P450scc, P450c17, 3βHSD and P450arom), spermatogenesis and cell proliferation were analyzed in the testes of fetal (164 to 218 days of fetal age), fawn (0 years old), yearling (1 year old) and adult (more than 2 years old) male sika deer. Three kinds of steroidogenic enzymes, P450scc, P450c17 and 3βHSD, essential for the synthesis of testosterone were located only in Leydig cells of the testes from the fetal period, and these localizations did not change during developmental or seasonal stages. Immunoreactivity for P450arom, a key enzyme converting testosterone to estradiol, was also localized only in the Leydig cells of testes but was also further limited to the testes of yearlings and adults. Seminiferous tubules had already formed in the fetal testes examined in the present study. Spermatogenesis started in yearlings and was more active in the breeding season. In the adult sika deer testes, the Leydig cells, which displayed immunoreactivities for steroidogenic enzymes, changed to have more cytoplasm in the breeding season than in the non-breeding season. Cell proliferation of Leydig cells was hardly observed in adult testes during seasonal changes. The present results suggested that sika deer testes start to synthesize testosterone from the fetal period, that seasonal changes in testosterone and estradiol syntheses are dependent on the quantitative variation of steroidogenic enzymes synchronized with the size of Leydig cells and that estradiol synthesized in yearling and adult testes makes a contribution to the initiation and recrudescence of spermatogenesis and spermiogenesis in the sika deer.
著者
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伊吾田 宏正
Npo法人 西興部村猟区管理協会
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鈴木 正嗣
北海道大学大学院獣医学研究科生態学教室
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Suzuki Masatsugu
北海道大学 獣医学研究科野生動物学
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鈴木 正嗣
北大 歯
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鈴木 正嗣
北海道大学・歯学部・第1解剖学講座
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Tsubota Toshio
Department Of Environmental Veterinary Science Graduate School Of Veterinary Medicine Hokkaido Unive
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Suzuki M
北海道大学大学院獣医学研究科生態学教室
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鈴木 正嗣
北海道大学歯学部
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Sasaki Motoki
Laboratory Of Veterinary Anatomy Department Of Basic Veterinary Medicine Obihiro University Of Agric
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Sasaki Motoki
Department Of Basic Veterinary Sciences Obihiro University Of Agriculture And Veterinary Medicine
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Sasaki Motoki
Laboratory Of Veterinary Anatomy Obihiro University Of Agriculture And Veterinary Medicine
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Kaji Koichi
Laboratory Of Wildlife Conservation Division Of Ecosciences Institute Of Symbiotic Science And Techn
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KITAMURA Nobuo
Laboratory of Anatomy, Department of Basic Veterinary Sciences, Obihiro University of Agriculture an
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Hayakawa Daisuke
Laboratory of Anatomy, Department of Basic Veterinary Science, Obihiro University of Agriculture and
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梶 光一
東京農工大学大学院共生科学技術研究院
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Hayakawa Daisuke
Laboratory Of Veterinary Anatomy Department Of Basic Veterinary Medicine Obihiro University Of Agric
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Hayakawa Daisuke
Department Of Anatomy Gifu University School Of Medicine
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Suzuki Masatsugu
Laboratory Of Wildlife Biology Graduate School Of Veterinary Medicine Hokkaido University
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Suzuki Masatsugu
Graduate School Of Veterinary Medicine Hokkaido University
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Suzuki Masatsugu
Laboratory Of Wildlife Biology Department Of Environmental Veterinary Science Graduate School Of Vet
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Suzuki Masatsugu
Laboratory Of Wildlife Biology Department Of Environmental Veterinary Sciences The Graduate School O
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Matsuzaki S
Genetics Hokkaido Inc. Hokkaido
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Sasaki M
Laboratory Of Veterinary Anatomy Department Of Basic Veterinary Medicine Obihiro University Of Agric
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SUZUKI Masatsugu
United Graduate School of Veterinary Medicine, Gifu University
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IGOTA Hiromasa
Department of Biosphere and Environment Sciences, Rakuno Gakuen University
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Igota H
Hokkaido Univ. Sapporo Jpn
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Igota Hiromasa
Department Of Biosphere And Environment Sciences Rakuno Gakuen University
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Suzuki M
Hokkaido Univ. Sapporo Jpn
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Tsubota Toshio
Department Of Basic Veterinary Science The United Graduate School Of Veterinary Sciences Gifu Univer
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Sasaki Motoki
Obihiro Univ. Agriculture And Veterinary Medicine Obihiro Jpn
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Suzuki M
Gunma Univ. School Of Medicine Maebashi Jpn
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伊吾田 宏正
酪農学園大学・npo法人西興部村猟区管理協会
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Suzuki Masatsugu
Laboratory Of Wildlife Biology The Graduate School Of Veterinary Medicine Hokkaido University
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鈴木 正嗣
岐阜大学・応用生物科学部
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Sasaki Motoki
Laboratory Of Anatomy Obihiro University Of Agriculture And Veterinary Medicine
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SUZUKI Masatsugu
Department of Oral Anatomy I, School of Dentistry, Hokkaido University
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SASAKI Motoki
Laboratory of Veterinary Anatomy, Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080–8555, Japan
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